P-169: Protective Effect of Vitamin E on Cypermethrin - Induced Damages Correlates with P53 Gene Expression and Nitrosative Stress
Authors
Abstract:
Background: Because of fast rate of degradation and low mammalian cells toxicity the cypermethrin (CPM) is a wide used insecticide in domestic agriculture and in veterinary medicine applications. The compound exerts its pathological impact by down-regulating the antioxidant status. Therefore, current study was designed to evaluate the protective effect of vitamin E on CPMinduced apoptosis and nitrosative stress on ovarian tissue. Materials and Methods: Eighteen Mature female rats were divided into three (N=6) test and control-sham groups. The animals in test group 1 received CPM (75 mg/kg) and in test group 2 received vitamin E (150 mg/ kg) + CPM by gavages, for 24 constitutive days. The normal saline was administrated in control-sham group. After evaluating the total RNA content the P53 gene expression was evaluated by RT-PCR. The tissue total protein (TP), total antioxidant capacity (TAC) and nitric oxide (NO) were examined. Results: Vitamin E administrated animals were manifested with significantly (p<0.05) higher total RNA (3986.33 ± 24.79) and protein (3.25 ± 0.03) contents versus the CPM alone-administrated (1847.34 ± 19.94 for RNA and 2.79 ± 0.12 for protein) group. The P53 gene expression was detected in CPM alone group while it was not observed in vitamin E-administrated group. The vitamin E up-regulated TP and TAC and reduced NO level compared to CPM alone group. Conclusion: Our data suggest that CPM impact the ovarian tissue by inducing the nitrosative stress which promotes the P53 gene which in turn leads to cellular apoptosis. On the other hand vitamin E by up-regulating antioxidant status inhibited the damages.
similar resources
P-66: Protective Effect of Testosterone and Vitamin E on Atrazine-Induced Toxicity in Testis
Background: Atrazine (ATR) is herbicide which is known to induce endocrine disruption. According to previous reports, long-time exposure to ATR leads to severe seminiferous tubules degeneration and significant reduction in sperm content. Moreover this chemical exerts its pathological impact partly by inducing oxidative stress. Therefore present study was designed in order to evaluate protective...
full textAmeliorative Effect of Vitamin E on Cypermethrin Induced Hepatotoxicity and Oxidative Stress in Male Wistar Rats
The present study was carried out to investigate the ameliorative effect of vitamin E on Cypermethrin induced hepatotoxicity and oxidative stress. For this purpose, a total of 24 rats were randomly divided into four equal groups: group I used as control and groups II, III and IV were orally treated with Cypermethrin (25 mg/kg body weight), Vitamin E (100 mg/kg body weight) and Cypermethrin (25 ...
full textProtective effect of vitamin E on cypermethrin-induced follicular atresia in rats ovary: Evidence for energy dependent mechanism
It has been shown that chronic exposure to cypermethrin (CPM), a pyrethroid pesticide, results in follicular atresia via pathologically affecting angiogenesis, disrupting endocrine potential and enhancing oxidative stress. This study was aimed to uncover the CPM-exposed energy dependent follicular cells apoptosis and to estimate protective effect of vitamin E (VitE) as a potent antioxidant. Thi...
full textProtective and synergistic effects of vitamin E and selenium against tissue and oxidative damages induced by cypermethrin in the rat’s testis
Background: Cypermethrin is an insecticide that is widely used in pest control programs. Previous studies have pointed out its harmful effects on the male reproductive system. Therefore, in the present study, vitamin E and selenium were used to protect the testicles from the damage of this poison. Materials and methods: For this study, 42 adult male Wistar rats were prepared and divided into 6...
full textP-61: Comparing The Protective Effects of Hydro-Alcoholic Extract of Cornus Mas Versus Vitamin E on Methotrexate-Induced Damages on Sperm Parameters
Background: Methotrexate (MTX), as antimetabolite and antifolate compound, is used to treat the certain types of tissue over activity and cancers. The MTX interferes with cell division through inhibiting the purine and pyrimidine synthesis. Moreover, the toxic impact of the MTX on gonadal tissues has been reported. Therefore, current study was designed to evaluate the protective effects of corn...
full textP-8: Protective Effect of Royal Jelly versus Vitamin C against Anemia-Induced Damages in Testicular Tissue
Background: Phenylhydrazine (PHZ) is used to induce experimental anemia in animal models. On the other hand royal jelly is known as antioxidant compound. Therefore, here in present study we aimed to evaluate the protective effect of royal jelly on PHZ-induced damages in testicular tissue and compare its impact with vitamin C. Materials and Methods: Eighteen mature male mice were randomly divide...
full textMy Resources
Journal title
volume 7 issue 3
pages 101- 101
publication date 2013-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023